Almost every fifth buffalo and cow in Pakistan is having mastitis. The main cause of this disease is the bacteria that enter the animal through the holes of the udder and make their way into the tissues. These bacteria and their excretions causes harm to the animal and reduce the production of milk and also have adverse effects on its quality. Cattle breeders can self-diagnose this disease with a simple surf test. The milk of an animal that is diagnosed with the disease is not suitable for human consumption, so the infected animal should be treated by a veterinary doctor in a veterinary hospital.